This repository contains tabular data files from Tn-Seq experiments performed on the Staphylococcus aureus HG003 transposon library under different treatment conditions (± Congo red or tunicamycin). These datasets were used for transposon sequencing (Tn-Seq) analysis and can be processed according to the methods described in the following publication:
Santiago et al., https://doi.org/10.1186/s12864-015-1361-3
Note: For easier navigation, view the files in Tree view to see the directory structure organized by treatment condition.
 
Folder: CongoRed_tabulars/
•   The HG003 transposon library was cultured in TSB medium ± 16 μg/mL Congo red at 37 °C until reaching OD₆₀₀ = 1.0.
•   Samples were prepared for next-generation sequencing and divided into six sub-libraries, each representing different transposon constructs.
•   Files with filenames containing Congored37 correspond to Congo red-treated samples.
•   Files containing untr refer to untreated control samples.
 
Folder: Tunicamycin_tabulars/
This folder contains data from tunicamycin-treated and untreated samples organized as follows:
•   Set1_Tun/, Set2_Tun/, Set3_Tun/: Data from three independent samples treated with 1 μg/mL tunicamycin.
•   Set1_2_untreated/: Control data used for both Set1_Tun and Set2_Tun.
•   Set3_untreated/: Control data used for Set3_Tun.
Experimental conditions:
•   The HG003 library was grown in TSB ± 1 μg/mL tunicamycin at 37 °C to an OD₆₀₀ of 1.0.
•   All treated and untreated sets, except Set1_Tun, include six sub-libraries, each representing distinct transposon constructs.
•   The Set1_Tun folder contains data from only one sub-library.
 
